Nested Polymerase Chain Reaction (Nested-PCR) As A Diagnostic Technique for Intestinal Helminth Infection
نویسندگان
چکیده
منابع مشابه
Diagnosis of canine coronavirus infection using nested-Polymerase Chain Reaction (n-PCR) by A. Pratelli; P0128.0899
Canine coronavirus (CCV) is a single-stranded RNA virus belonging to the Coronaviridae family and is responsible for generally mild gastroenteritis in pups. Infected dogs shed CCV in feces for 6-9 days, but shedding can be prolonged in some pups. The virus content of feces is very high at the time of clinical signs. Electron microscopic (EM) examinations of fecal suspensions or isolation in tis...
متن کاملThe use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients*
The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8)....
متن کاملSpecies-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 stra...
متن کاملUnusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction
High mortality of nestling cockatiels (Nymphicus hollandicus) was observed in one breeding flock in Slovakia. The nestling mortality affected 50% of all breeding pairs. In general, all the nestlings in affected nests died. Death occurred suddenly in 4to 6-day-old birds, most of which had full crops. No feather disorders were diagnosed in this flock. Two dead nestlings were tested by nested PCR ...
متن کاملPolymerase Chain Reaction (PCR)
The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI
سال: 2020
ISSN: 2355-8059,2087-9725
DOI: 10.36722/sst.v5i4.427